Journal: Parasite Immunology
Article Title: Human filarial Wolbachia lipopeptide directly activates human neutrophils in vitro
doi: 10.1111/pim.12122
Figure Lengend Snippet: Wo LP (0·1 μg/mL) induces upregulation of integrins (CD11b and CD18) and shedding of L-Selectin on isolated neutrophils, similar to the effects of GM-CSF. * P = 0·025 for Wo LP and P = 0·033 for GM-CSF; ** P = 0·003; *** P ≤ 0·001. Bar graph represents percentage change (mean ± SD) in mean fluorescence intensity compared with stimulation with control Dimethyl Sulfoxide (DMSO) (for WoLP) and media alone (for GM-CSF) of neutrophils from n = 5 donors.
Article Snippet: Briefly, neutrophils were stained for 30 min on ice in FC buffer (0·2% Bovine Serum Albumin (Sigma Aldrich) in PBS) with FITC-conjugated rat antihuman CD11b IgG2 b (Miltenyi Biotec, Bisley, UK), mouse antihuman CD18 IgG 1 (R&D Systems, Minneapolis, MN, USA), mouse antihuman L-Selectin IgG 1 (R&D Systems) and mouse IgG 1 isotype control (Santa Cruz Biotechnology Dallas, TX, USA).
Techniques: Isolation, Fluorescence